Not yet convinced of gene synthesis? Read the latest success story on my blog. Very special thanks to Felix List. SDS-PAGE analysis of wild-type expression (1) compared to codon optimized gene (2), both in E.coli expression strain BL21 (DE3) Rosetta2 using pET24a. (3) Protein Standard. Histograms on the right show codon quality distribution in wildtype (1) and optimized gene (2). No bad codons (labeled red) are in the optimized synthetic gene (2). |