and Mr. Gene did a great job in simplifying the sequences and synthesizing the optimized genes. Other vendors were either unable to simplify the sequences, or charged a lot more than Mr. Gene.
Tian Chi, M.D., Ph.D., Yale School of Medicine / USA
was prepared by Mr Gene while other companies refused to synthesize the gene due to a very high GC content.
Prof. Dr. Bechthold, Pharmaceutical Biology, Albert-Ludwigs-Universität, Freiburg / Germany
for me to use gene synthesis. I needed codon optimization because as structural biologist I am aiming for high expression levels. And indeed: The gene performed well - expression levels in E.coli are very good. In addition, Mr. Gene's website is so easy to use. I didn't think that cloning could be so simple!
Claus D. Kuhn, Ph.D, Cold Spring Harbor Laboratory - Joshua-Tor Lab / USA
that codon optimization can boost protein expression that much. Thanks to Mr. Gene’s optimized sequence we save a lot of time and money during protein expression and purification. The online step-by-step process made it easy to design, optimize and order my gene without waiting. This is perfect when you want to get started.
Felix List, Physical Biochemistry, University of Regensburg / Germany
to have this fast open access source for synthetic genes. For most genes needed in research, reliability and a good cost-value ratio is important. Gene optimization and ordering is done online in a self explaining way in a few minutes.
Prof. Dr. P. Hegemann, Experimental Biophysics, Humboldt-University / Berlin / Germany
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